||The basic principles of spectrophotometry and the operation of a spectrophotometer are explained.
||The transmittance and absorbance are measured for a sample. A blank is used to calibrate the spectrophotometer.
||The absorbance spectrum is described and its importance explained.
||The absorbance spectrum for a sample is measured.
|Effect of Cell Path Length
||The significance of the cell path length is discussed.
||The effect of the cell path length on transmittance and absorbances is explored.
|Effect of Concentration
||The significance of the analyte concentration is discussed.
||The effect of the analyte concentration on transmittance and absorbances is explored.
||Beer's Law is presented and explained.
||A plot of absorbance vs concentration is prepared, and the molar absorptivity is determined from the slope of the plot.
|Analysis of an Unknown Solution
||The construction of a calibration curve is described, and the use of a calibration curve in determining the analyte concentration in an unknown solution is explained.
||A calibration curve is prepared and used to determine the analyte concentration in an unknown solution.