Solutions to Web Experiment:Spectrophotometry
Web Experiment: Spectrophotometry
Answers to Questions
- The absorbance or percent transmittance measurements should be made at 410 nm. If other chemical species in the analytical solution absorb at 410 nm, then 575 nm would be a suitable wavelength.
- The percent transmittance and absorbance measurements made at 410 nm are listed below.
| Standard Cr(III) Solutions |
%T |
A |
| 0.0100 M |
66.0 |
0.180 |
| 0.0200 M |
43.7 |
0.360 |
| 0.0300 M |
29.3 |
0.532 |
| 0.0400 M |
20.2 |
0.694 |
- A calibration curve was constructed with the aid of the Linear Regression applet. The plot of absorbances (y = A) versus the molarities of the standard Cr(H2O)63+ solutions ( x = c = [Cr(III)] ) is a straight line. The Cr(H2O)63+ solutions comply with Beer's law.
- The percent transmittance and absorbance of unknown #1 were measured at 410 nm and are listed below.
| Unknown |
%T |
A |
| #1 |
23.1 |
0.637 |
Note that the intercept for the plot of A versus [ Cr(H2O)63+] is not zero.