Solutions to Web Experiment:Spectrophotometry

Web Experiment: Spectrophotometry
Answers to Questions



  1. The absorbance or percent transmittance measurements should be made at 410 nm. If other chemical species in the analytical solution absorb at 410 nm, then 575 nm would be a suitable wavelength.



  2. The percent transmittance and absorbance measurements made at 410 nm are listed below.

    Standard Cr(III) Solutions %T A
    0.0100 M 66.0 0.180
    0.0200 M 43.7 0.360
    0.0300 M 29.3 0.532
    0.0400 M 20.2 0.694


  3. A calibration curve was constructed with the aid of the Linear Regression applet. The plot of absorbances (y = A) versus the molarities of the standard Cr(H2O)63+ solutions ( x = c = [Cr(III)] ) is a straight line. The Cr(H2O)63+ solutions comply with Beer's law.











  4. The percent transmittance and absorbance of unknown #1 were measured at 410 nm and are listed below.

    Unknown %T A
    #1 23.1 0.637


    Note that the intercept for the plot of A versus [ Cr(H2O)63+] is not zero.